The separated proteins had been transferred to a PVDF membrane using 10% SDS-PAGE. Protein detection was carried out utilizing equal volumes of ECL options A and B (UU-Bio technology, U10012, Zhousu, China). The in vitro assay was modified from an present protocol from the previous report [44]. We used one hundred mM citrate buffer solution (pH 6) with 10 mM of ZnSO4.7H2O and zero.2% w/v NaN3 as a buffer resolution. In the preparation step, all samples from the library had ...
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